Journal: Molecules

Article Title: nurP28, a New-to-Nature Zein-Derived Peptide, Enhances the Therapeutic Effect of Docetaxel in Breast Cancer Monolayers and Spheroids

doi: 10.3390/molecules27092824

Figure Lengend Snippet: Monitoring breast cancer MCF7 spheroids treated with nurP28 peptide alone and in combination with DTX at 0, 24, 72, and 120 h. ( A ) Bright-field images of MCF7 spheroids exposed to the different treatments; ( B ) Glucose concentration (primary axis), and cytotoxicity (secondary axis), assayed by LDH, from culture media of the treated spheroids. Results expressed as mean ± SD ( n = 3). The letters a, b and c indicate a statistically significant difference at p < 0.01. Bars with no common letters are significantly different.

Article Snippet: We performed the fluorometric LDH-cytotoxicity assay (Abcam, Boston, MA, USA ab197004) to assess the degree of cell death on spheroids in response to the exposure to our treatments.

Techniques: Concentration Assay

Journal: Biomedicines

Article Title: Self-Organization Provides Cell Fate Commitment in MSC Sheet Condensed Areas via ROCK-Dependent Mechanism

doi: 10.3390/biomedicines9091192

Figure Lengend Snippet: Cells from condensed areas of the cell sheet (CS) are characterized by an increased content of the cytochrome-C oxidase IV component (COX-IV) and higher lactate dehydrogenase (LDH) activity. ( A ) Western blotting of the mitochondrial ETC COX-IV subunit in lysates of ML, total CS and microdissected condensed or scattered areas. Graphs present a densitometry analysis of the COX-IV content normalized to β-actin (densitometry analysis). ( B ) LDH activity (LDHact) in the lysates of microdissected condensed areas and scattered areas. DNA amounts in the respective cell lysates were used for the normalization of LDH activity to the cell number. Control data was obtained from lysates of the A431 cell line that increased the proportion of anaerobic glycolysis and the THP-1 cell line with the major input of oxidative phosphorylation but not glycolysis. The band numbers indicate individual biological replicate samples. In the figure graphs and histograms, significant differences were marked by * ( p < 0.05) and ** ( p < 0.005).

Article Snippet: The enzymatic activity of lactate dehydrogenase (LDH) in condensed and scattered areas of CS was assayed in the samples obtained immediately after laser microdissection using a Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000, Abcam, Cambridge, UK) according to the manufacturer’s instructions.

Techniques: Activity Assay, Western Blot